SARS-CoV-2 HMP – N1, N2 & RNase P Multiplex is a real-time reverse transcriptase, polymerase chain reaction (PCR) assay for multi-platform use on the BD MAX™, Bio-Rad CFX96 Touch™ and Applied Biosystems QuantStudio™ 5 platforms for the qualitative detection of the presence of RNA from nucleocapsid phosphoprotein gene (N gene) of the SARS-CoV-2 coronavirus and the human RNase P gene. The primer and probe sets are based on the United States Centers for Disease Control and Prevention (US CDC) assay for specific detection of SARS-CoV-2 by amplifying two unique regions of the N gene (i.e., N1 and N2). Detection of the human RNase P gene serves as an endogenous SampleProcessing Control (SPC).
The multiplex PCR assay is provided in BioGX Sample-Ready™ lyophilized format. Each tube contains all PCR components such as primers, probes, enzymes, dNTPs, MgCl, and buffers required for real-time PCR-based testing of 24 BD MAX™ samples or 48 samples analyzed on the Bio-Rad CFX96 Touch™ or Applied
Biosystems QuantStudio™ 5.